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Extra resources for Liposome Methods and Protocols (Methods in Molecular Biology Vol 199)
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And Kostetsky, E. Y. (1968) Modified spray for the detection of phospholipids on thin-layer chromatograms. J. Lipid Res. 9, 396. 22. , and Sonnino, S. (1994) Geometrical and conformational properties of ganglioside GalNAc-GD1a, IV4GalNAcIV3Neu5 AcII3Neu5AcGgOse4Cer. Eur. J. Biochem. 225, 271–288. 23. , and Tettamanti, G. (1984) High performance liquid chromatography preparation of the molecular species of GM1 and GD1a gangliosides with homogeneous fatty acid and long chain composition. J. Lipid Res.
3. Liposomes Composed of SM, Containing Domains of Cholesterol and of GM1 Ganglioside In these liposomes, distinct SM/cholesterol and SM/ganglioside domains coexist. The main characteristics of these liposomes are the following: size 100 nm (1000 Å); shape monolamellar; gel to liquid-crystalline temperature transition (Tm) 38°C, determined by high-sensitivity differential scanning Liposomes Containing Sphingolipids 23 calorimetry. Therefore, the liposomes are in the physical state of gel up to this temperature, and this feature should be taken into account anytime the physical state is important for the particular experiment.
Common lipids from both sources gave very similar results in terms of quality. Phospholipid standards and molybdenum blue spray reagent were obtained from Sigma. 2. Fluorescent lipid probes N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidyl ethanolamine (N-NBD-PE) and N-(lissamine rhodamine B sulfonyl)-dihexadecanoylsn-glycero-3-phosphoethanolamine (N-Rh-PE) were obtained either from Avanti Polar Lipids (Alabaster, AL) or from Molecular Probes (Eugene, OR). 3. Polyunsaturated phosphatidylcholine species were exclusively purchased from Avanti Polar Lipids (and used within 3 mo).