By Feroze N. Ghadially
Diagnostic Electron Microscopy of Tumors, moment variation is a advisor on how one can hire electron microscopy recommendations in making review on tumor biopsies. This variation goals to function a resource of knowledge, references, and electron micrographs for the working towards electron microscopists. This booklet is split into 5 components; each one of which tackles a unique subject. half 1 discusses the gathering of specimen, and half 2 talks concerning the overview of tumors' habit and development. Ultrastructural research of diagnostic difficulties is roofed partially three; the importance of a few ultrastructural gains in tumors is defined in elements four and five. this article is usually recommended if you desire to learn tumor biopsies with an electron microscope in addition to those that are looking to develop into extra conversant with such issues, in order to comprehend and overview electron micrographs and studies. This e-book can be helpful to physicians, surgeons, histopathologists, and pathology citizens.
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Extra info for Diagnostic Electron Microscopy of Tumours
Example text
One or more of these features is quite prominent in fast-growing malignant tumours but less apparent or absent in well-differentiated tumours and benign tumours. The idea that mitochondria in tumours are defective is also supported by many biochemical studies. , 1969; Sordahl et ai, 1969; Pedersen et al, 1970). The paradox of a fast-growing tissue with a paucity of mitochondria and/or defective mitochondria may be explained by recalling the historical work of Warburg (1956, 1962), which showed the predominance of glycolysis over respiration in the energy metabolism of tumours.
The swollen mitochondria (S) in the tumour cell are well contrasted from the normal-looking mitochondria (M) in the lymphocyte, x22000 Figure 54 Residual retroperitoneal leiomyosarcoma after radiation. Note mitochondria with swollen intracristal space and dense matrix. This is sometimes referred to as the 'condensed configuration', x28000 53 Figure 55 Chronic myeloid leukaemia. A myelocyte found in the peripheral blood. The mitochondria show a more Figure 56 Adenocarcinoma of the ovary. A tumour cell showing mitochondrial pyknosis.
Same specimen as Figure 50. The meandering nucleolus appears fragmented and scattered in the nucleus, x 12000 Nucleolar margination observed during the growth phase of this benign tumour (see Plate 26 in Ghadially, 1982). Irregularities of nuclear shape, invaginations of the nuclear envelope and pseudoinclusions are common features of the neoplastic nucleus, and in some rapidly growing malignant tumours such deep invaginations are seen to make contact with or penetrate the nucleolus (Figure 52).