By P. S. Gardner (auth.), M. S. McNulty, J. B. McFerran (eds.)
The conventional method of analysis of virus infectians by means of isolation of the causative virus is mostly either gradual and dear. extra lately, the emphasis has been at the direct detection of viruses or viral antigens in scientific specimens. this is performed utilizing confirmed recommendations reminiscent of immunofluorescence or electron microscopy, or by means of newly built biochemical tools. the aim of this assembly was once to check those and different advancements within the laboratory prognosis of virus infections. we want to thank all those that contributed to the luck of this assembly. specifically, we're thankful to the CEC for monetary sponsorship, to Professor C. Dow, Director of the Veterinary learn Laboratories, for assist in setting up the assembly, and to overlook B. Hamilton for her very good typing of the manuscripts. M S McNulty J B McFerran VII CONTENTS web page Preface V VIRAL prognosis by means of TIMMUNOFLUORESCENCE P. S. Gardner functions OF IMMUNOFLUORESCENCE IN VETERINARY VIRAL prognosis M. S. McNulty and G. M. Allan 15 TAGGING OF VIRAL ANTIGENS utilizing IMMUNOPEROXIDASE . AND IMMUNOOOLD TEGINlQUES R. Ducatelle, F. Castryck and J. Hoorens 27 DETECTION OF BVD VIRUS IN VlRAEMIC livestock via AN oblique IMMUNOPEROXIDASE TEGINIQUE A. Meyling 37 VIRAL prognosis by means of ELECTRON MICROSCOPY J. D. Almeida forty seven seize ASSAYS FOR THE DETECTION OF VIRUS-SPECIFIC IgM ANTIBODY R. S. Tedder 60 DETECTION . AND id OF PAPILLOMAVIRUSES IN BENIGN . AND MALIGNANT 'TIlmJRS OF farm animals M. S. Campo seventy two DETECTION OF VIRAL NUCLEIC ACIDS IN mobilephone CULTURES .
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Additional resources for Recent Advances in Virus Diagnosis: A Seminar in the CEC Programme of Coordination of Research on Animal Pathology, held at the Veterinary Research Laboratories, Belfast, Northern Ireland, September 22–23, 1983
Example text
A simple and rapid immunoperoxidase test for the detection of virus antigens in tissue culture. Vet. , 106, 506-507. D. F. 1982. Applications of peroxidase-antiperoxidase staining technique for detection of transmissible gastroenteritis virus in pigs. Am. J. Vet. , 43, 77-81. , Braidotti, P. and Coggi, ~ 1982. Immunohistochemical localisation of human immunoglobulins and Lysozyme in epoxy-embedded lymph nodes. J. Histochem. , 30, 630-636. A. M. 1973. Immunohistochemical demonstration of avian leukosis virus antigens in paraffin embedded tissue.
It should also be remarked that the relatively low speed used for centrifugation is an attempt to strike a balance between virus recovery on the one hand and avoidance of too much contaminating low molecular weight protein on the other. The pellet obtained from this preparatory operation is now ready for negative staining. Grids Electron microscope grids come in a range of mesh sizes and forms for 49 different specialist purposes. Since negative staining yields specimens that contain a wide range of three dimensional structures, they put considerable stress on the substrate and therefore only small mesh grids with a stable supporting film should be used.
A FITC-conjugated antiserum to bovine enteric coronavirus has been used to look for coronavirus antigens in necropsy material from sheep. So far, only a small number of ovine carcases has been available for examination. However, immunofluorescence has been detected in the cytoplasm of bronchiolar epithelial cells of a lamb with pneumonia. This provides further evidence for coronavirus infection in sheep. B. M. 1980. Development of a microtitre fluorescent antibody test for serological detection of adenovirus infection in birds.