By Harris Busch
The telephone Nucleus, quantity III: Nuclear debris, half A covers subject matters on "splicing" and "processing" and at the controls of transcriptional and shipping occasions that has to be necessary to cells which are both starting to be and/or are phenotypically differentiated. The ebook discusses the immunolocalization and structural association of nascent RNP; the in situ localization of RNA buildings; and the morphogenesis, cytochemistry, and putative function of perichromatin granules. The textual content describes transcription within the remoted nuclei; the isolation and constitution of the ribonucleoprotein fibrils containing heterogeneous nuclear RNA; and low-molecular-weight nuclear ribonucleoprotein debris. The series, functionality, and position of U snRNAs of nuclear snRNPs; the maturation of low-molecular-weight RNA species; and the homes of the heterogeneous nuclear RNA-protein complexes and nuclear matrix also are encompassed. The publication extra tackles human antibodies to RNA-containing debris. mobile biologists, microbiologists, pharmacologists, geneticists, and scholars taking comparable classes will locate the e-book priceless.
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Extra resources for Nuclear Particles. The Cell Nucleus, Volume 8
Sample text
1975) their source and possible function remain unclear. The apparent presence of tubulin on lampbrush loops might, like histone, result from adsorption of accumulated protein, but whether a general case can be made for the loops acting as repositories for such proteins will have to be tested formally. 2 . LABELING OF SPECIFIC LOOPS Of the several antibodies raised against protein fractions of oocyte nuclear RNP only one has been found to react with a restricted number of transcriptionally active loci (loops).
Heavily transcribed chromatin intercepts such as the nucleolar genes from a large variety of organisms (for reference, see Franke et al, 1977), lampbrush loop axes of amphibian oocytes (Miller and Bakken, 1972; Angelier and Lacroix, 1975; Scheer et al, 1976, 1979b; Hill, 1979; Scheer and Sommerville, 1980) and of the primary nuclei of Acetabularia (Spring et al, 1974, 1975; Scheer et al, 1976), and fibroin genes in silk gland cells of Bombyx mort (McKnightef al, 1976) carry lateral RNP fibrils with a frequency in the range of 20-50 fibrils/μ,ιη chromatin axis.
Most of these studies have been concentrated on either the location of individual histones in polytene chromosomes (Desai et al, 1972; Alfagame et al, 1976; Jamrich et al, 1977; Silver and Elgin, 1977; Bustin et al, 1977; Kurth et al, 1978) or the involvement of different histones in conformational states of chromatin (Bustin et al, 1976a, 1977; Goldblatt et al, 1978). , 1976; Silver and Elgin, 1976, 1977, 1978). However the non-histone proteins studied in these experiments are believed to function in the regulation of genetic activity by binding to chromosomal DNA, presumably at specific sequences (Alfagame et al, 1976; cf.