By Lawrence Bogorad

The Photosynthetic gear: Molecular Biology and Operation.

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Extra info for The Photosynthetic Apparatus: Molecular Biology and Operation. Cell Culture and Somatic Cell Genetics of Plants

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Shaw, E. , and Ke, B. (1966). A photochemically active particle derived from chloroplasts by the action of the detergent Triton X-100. /. Biol. Chem. 241, 4101-4109. , and Ochoa, S. (1951). Photochemical reduction of pyridine nucleotides by spinach grana and coupled carbon dioxide fixation. Nature 167, 768-769. Wassink, E. C , and Kersten, J. A. (1946). Observations sur le spectre d'absorption et sur le role des carotenoides dans la photosynthese der diatomees. Enzymologia 12, 3-32. , and Osborn, M.

PS II-B, PS II-C, and light-harvesting chlorophyll alb protein (LHC-II) are all chlorophyll proteins of PS II. PS I-A, PS I-B, and LHC-I are chlorophyll proteins of PS I. All the proteins shown are products of plastid genes except those that are cross-hatched. The latter are products of nuclear genes. PS II-J, the presumed product of psb], has not been detected in PS II preparations. PS II-G may be a part of an unidentified dehydrogenase complex rather than a component of PS II. See Chapters 2B, 4B, and 5B for additional information.

The material in each zone was analyzed by SDS-PAGE and the two green fractions were found to contain some uniquely migrating polypeptides, although many other polypeptides appeared to be common, perhaps from cross contamination. Levine et al. (1972) obtained comparable results with fractions of spinach thylakoids produced by the methods of Boardman and Anderson. , Apel et al, 1975, 1976; Chua and Bennoun, 1975; Henriques and Park, 1975) as many as 50 or so polypeptides ranging in molecular weight from about 11 to 120 kDa could be distinguished on polyacrylamide gradient gels of total thylakoid membranes completely dissociated with SDS.

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