By Agnes M. Rimando and Scott R. Baerson (Eds.)
Content material: A plethora of polyketides: constructions, organic actions, and enzymes / S. R. Baerson ... [et al.] -- Hygromycin a biosynthesis / N. Palaniappan ... [et al.] -- The biosynthesis of polyketides, tetramic acids, and pyridones in fungi / R. J. Cox -- looking for polyketides in insect pathogenic fungi / D. M. Gibson ... [et al.] -- Polyketide biosynthesis in fungi / okay. C. Ehrlich -- Biochemical and molecular research of the biosynthesis of fumonisins / L. Du ... [et al.] -- Benzoic acid-specific variety III polyketide synthases / L. Beerhues ... [et al.] -- Engineered biosynthesis of plant polyketides: Chain size regulate in novel variety III polyketide synthases / I. Abe -- Expression and serve as of fragrant polyketide synthase genes in raspberries (Rubus idaeus) / G. Hrazdina ... [et al.] -- Molecular and biochemical characterization of novel polyketide synthases more likely to be all for the biosynthesis of sorgoleone / D. prepare dinner ... [et al.] -- Polyketide biosynthesis past the kind I, II, and III polyketide synthase paradigms: A development record / B. Shen ... [et al.] -- Structural enzymology of fragrant polyketide synthase / T. P. Korman ... [et al.] -- Mechanisms of variety iii polyketide synthase sensible range: From 'steric modulation' to the 'reaction partitioning' version / M. B. Austin ... [et al.] -- Novel polyketides from genetic engineering (... and classes we have now discovered from making them) / L. Katz ... [et al.] -- Biosynthesis of the antifungal polyketide antibiotic soraphen a in Sorangium cellulosum and Streptomyces lividans / I. Molnar ... [et al.] -- Engineering starter devices in fragrant polyketides / W. Zhang ... [et al.] -- Aspergillus flavus genomics for locating genes fascinated by aflatoxin biosynthesis / J. Yu ... [et al.]
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Extra info for Polyketides. Biosynthesis, Biological Activity, and Genetic Engineering
Example text
As shown in Figure 5A, the H P L C analyses of S. hygroscopicus N R R L 2388 clearly reveals the presence of hygromycin A (1) and methoxyhygromycin A (2) (the 5" epimeric forms of these two compounds appear as smaller shoulder peaks at a slightly earlier retention time). Production levels under the fermentation conditions used were typically 350 mg/L of methoxyhygromycin A , and 880 mg/L of hygromycin A . In contrast to the wild type strain, the SCH30 mutant generated no detectable levels of hygromycin A or methoxyhygromycin A , but three new peaks of hygromycin A analogs were observed (3, 4 and 5 in Figure 5B).
Larke, L . ; Murphy, L . ; Oliver, K . ; Rajandream, M . A . ; Rutherford, K . ; Seeger, K . ; Taylor, K . ; Wietzorrek, A . ; Barrell, B . ; Hopwood, D. A . Nature 2002, 417, 141. 35. ; Ikeda, H . ; Hanamoto, A . ; Shinose, M . ; Takahashi, Y . ; Horikawa, H . ; Nakazawa, H . ; Kikuchi, H . ; Sakaki, Y . ; Hattori, M . Proc. Natl. Acad. Sci. USA. 2001, 98, 12215. 36. Barrasa, M . ; Tercero, J. A . ; Jimenez, A . Eur. J. Biochem. 1997,245, 54. 37. ; Dehesh, K . ; Lindqvist, Y . , EMBOJ. 1998, 17, 1183.
4 5 0 Other products of fungal P K S N R P S - Relationships and Hypotheses. Intriguingly L N K S , one of the best investigated fungal polyketide synthases has long been known to possess NRPS domains (2). Similarly to FUSS and TENS, it contains a seemingly intact condensation domain after the P K S A C P , and the JV-terminal region of the adenylation (A) domain. ; ACS Symposium Series; American Chemical Society: Washington, DC, 2007. ch003 43 X X Scheme 2. Proposed reactions catalysed during the biosynthesis of tenellin 19.