By Edward Ford
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Additional resources for Observations on the disease of the hip joint: To which are added, some remarks on white swellings of the knee, the caries of the joint of the wrist, and ... engravings, taken from the diseased parts
Sample text
Used an expression profile compendium including the mRNA profiles of 215 strains carrying down-regulatable promoters upstream of essential genes as well as 300 additional profiles [38, 46]. The validity of their approach was proven by predicting the thioredoxin–thioredoxin reductase system as the target of the mechanistically uncharacterized anti-cancer compound (1-phenyl-1H-tetrazol-5-ylsulfonyl-butanenitrile; PTSB) which was subsequently independently confirmed. In contrast to yeast, however, the experimental handling of conditional mutants under-expressing essential genes is non-trivial for many bacteria.
Undoubtedly, such studies will have a major impact on our current understanding of the physiology of microbial pathogens, the human host’s immune response to infection, as well as the effect of chemotherapy on the infectious agent and the host. This, in turn, will become a solid basis for discovering and developing innovative antimicrobials for combating infectious disease. Transcriptional profiling will undoubtedly continue to play an indispensable role in this process. Acknowledgements We are grateful to N.
A schematic overview of the processes in which transcription profiling technologies are being used successfully or for which the potential utility of microarrays has been demonstrated. This includes (1) target identification and validation, (2) efficacy mechanismof-action (MOA) characterization of drug candidates, (3) development of novel types of drug screening assays, and (4) prospective drug safety and toxicology studies (see text). can be spotted on both types of arrays, oligonucleotides are generally immobilized on glass slides.