By Karl Maramorosch
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Extra info for Mycoplasma Diseases of Trees and Shrubs
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Vein clearing is a common symptom on leaves in broomed twigs, particularly in the early stages. If brooming is severe the leaves may be come narrowed at the base. Because brooms usually do not form until late summer, they continue growth into late fall or early winter and commonly die back during the winter. , 1942). , 1942). No vector is known. 1 1 Fig. 3. Mycoplasma-like bodies in the sieve elements of black locust (1) appear to be moving through sieve plates; mitochondrion (2); P-protein (3); and crystalline protein (4) .
Spiroplasma DNA has been characterized also by a very re cently developed technique that is based on fragmentation of the nucleic acid molecule by restriction endonuclease and sub sequent separation of the fragments by polyacrylamide gel electrophoresis. However, the use that may be made of this highly sensitive technique for taxonomic purposes is limited by the fact that, although the endonuclease restriction frag ment patterns of some strains withing a given spiroplasma species or serogroup are quite similar, others are surprising ly different (Bove and Saillard, 1979).
Wilson granular while the larger bodies contained central fibullar material and a granular peripheral zone. All cells were bound ed by a unit membrane approximately 10 nm thick. Several cells, apparently in the process of budding, were observed with a double membrane. A witches -broom on Arizona ash, which is identified as Fraxinus berlandierana A. D C , was first reported in Louisiana by Plakidas (1949). Later, a similar brooming disease was al so reported in Louisiana on green ash, F. pennsylvanica Marsh.