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Sample text
The user selects an intensity threshold, where pixels that have a value above the threshold are presumed to represent cells and pixels that have a value below the threshold are presumed to represent background. Locally adaptive thresholds, texture-based segmentation and other sophisticated schemes allow this segmentation to adapt to varying background intensity levels, texture and other effects (Russ, 1995). Next, islands of segmented sections are identified and labeled as separate objects as illustrated in Figure l-18c.
The randomness requirement implies that the particles being analyzed must be randomly positioned. If not, the probe, described later, must be randomly positioned. The independence requirement implies that separate image fields that are analyzed must be independent from one another such that overlapping areas containing the same particles are avoided. Stereology is a broad field which historically has been used primarily in studying materials by electron microscopy. It became necessary for that field, because 3-D image sets of sufficient size for gathering true 3-D morphometry are not common.
Effect of charge integration time on signal-to-noise with a cooled CCD camera. Charge integrations were of (Left) 10msec, (Middle) 30msec, and (Right) lsec. A similar effect would be seen with frame averaging. With a low SNR per frame, then, this example depicts an equivalent situation of having 1 frame, 3 frames averaged and 100 frames averaged, respectively. and magnification. Electronic signal noise in the camera also contributes to the snow-like noise seen in Figure l-12a. This type of noise originates from random release of electrons in the photosensitive semiconductor CCD screen and from the amplifier electronics that are interfaced to this CCD plate.