By Joanne T. Douglas, David T. Curiel (auth.), Prof. Robert A. Stockley (eds.)
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Extra resources for Molecular Biology of the Lung: Volume II: Asthma and Cancer
Sample text
Barnes et al. [30] examined two groups for linkage of chromosome 12 markers with asthma and total serum IgE: the first consisted of29 AfroCaribbean multiplex families derived by recruiting the extended families of 29 asthmatic probands, and the second consisted of the 11 Amish kindreds described by Marsh et al. [5] plus 39 additional members, including one new family. 01) although at a lower level of significance. 003). This is 13 cM downstream from the IFNG gene and 14 cM upstream from the IGFI gene, effectively ruling them out as candidates for the locus affecting these two phenotypes.
1 . Transcription Factor Families Several families of transcription factors exist and members of each family may share structural characteristics. These families include helix-turnhelix (e. g. g. g. HU) [5]. Many transcription factors are common to several cell types (ubiquitous), whereas others are cell specific and determine the phenotypic characteristics of a cell . 2. Methods for Studying Transcription Factors There is relatively little information about the regulation of transcription factors in the airways, particularly in diseases such as asthma.
To date there is no confirmation of many of these results, and the number of subjects has generally not approached that required to establish an unequivocal HLA association. In addition, there has been no recognition of the problems of reactivity to multiple allergens: significant relationships between HLA-DR alleles and five antigens (Amb a V, Lo! pI, Lo! p II, Lol p III and Amb a VI) have been claimed from the same pool of about 200 subjects [34, 36, 37,40]. To test more definitively whether HLA class II gene products have a general influence on the ability to react to common allergens, we have genotyped for HLA-DR and HLA-DP in a large sample of atopic subjects from the British population [44].