By Min Gu, Damian Bird, Daniel Day, Ling Fu, Dru Morrish
The advent of femtosecond pulse lasers has supplied quite a few new tools for non-destructive diagnostic research of organic samples. This ebook is the 1st to supply a targeted and systematic remedy of femtosecond biophotonic tools. each one bankruptcy combines conception, perform and purposes, strolling the reader via imaging, manipulation and fabrication suggestions. starting with an evidence of nonlinear and multiphoton microscopy, next chapters tackle the strategies for optical trapping and the improvement of laser tweezers. In a end that brings jointly a few of the subject matters of the ebook, the authors talk about the transforming into box of femtosecond micro-engineering. the wide variety of functions for femtosecond biophotonics capability this publication will attract researchers and practitioners within the fields of biomedical engineering, biophysics, existence sciences and medication.
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Additional info for Femtosecond Biophotonics: Core Technology and Applications
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Since the fluorescence light excited by scattered photons quickly becomes dominant if d > ls , the near diffraction-limited image is often overshadowed by the blurred part of the image. However, because of the quadratic dependence of fluorescence light on the excitation intensity under two-photon excitation, the effect of ballistic photons on the sharp part of an image is greatly enhanced. Therefore the two overlapped parts can be clearly observed in Figs. 5(c) for an optically thick turbid medium.
8 ns. EGFP expressed in cells was predominantly cytoplasmic, and so fluoresced only weakly in the centre of the cell. 69 ns (Fig. 17) averaged from three spectra at different spots was much shorter than the two-photon extracellular lifetimes (Fig. 17(a)). 2. 17(b) shows the mapping of the lifetimes, indicating the spatial dependence of the lifetime within a cell. 6 ns) was much shorter than the two-photon extracellular lifetimes. It is known that EGFP is highly susceptible to forming dimers. It is suspected that it is the formation of these dimers that causes the shortened lifetime we observe for cellular EGFP.