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Punctata 37 was fully susceptible. Aeromonas isolates 37 and 42 were resistant to nalidixic acid (MIC >256 mg/L) and to fluoroquinolones (Table). However, A. media 42 exhibited higher resistance levels to fluoroquinolones, with MICs 2- to 8-fold higher than those for A. punctata 37 (Table). Sequence analysis of the QRDR regions of gyrA and parC genes showed that A. punctata 37 had 1 aa substitution, Ser83Ile in GyrA, whereas A. media 42 had 2 aa substitutions, Ser83Ile in GyrA and Ser80Ile in ParC, as compared with the wild-type proteins of Aeromonas species (18).

Coli TOP10 and azide-resistant E. coli J53 strains were recipient strains for transformation and conjugation experiments, respectively (20). Conjugation experiments were performed at 22°C and 37°C, as previously described (21). Transformants and transconjugants were selected on MH agar plates containing nalidixic acid (3 mg/L) only or containing azide (100 mg/L) plus nalidixic acid (6 mg/L), respectively. Plasmid extraction was performed from each qnr-positive isolate and its corresponding transformants by using the Kieser technique (22).

Gov/eid • Vol. 14, No. 2, February 2008 Genetic Determinants of WNV Virulence and the presence of neuroinvasive strains, may reflect inadequate surveillance and a lack of medical awareness of the disease potential of arboviruses. Moreover, the importance of WNV in South Africa may be overshadowed by the presence and effect of other diseases such as HIV/AIDS. Nevertheless, the epidemic potential and effect that WNV may have on a large population of immunocompromised HIV-infected persons necessitates improved surveillance of arbovirus infections of persons in southern Africa.

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