By John Kuo

This publication offers the most recent expertise in electron microscopy. It contains significant parts of electron microscopy - transmission electron microscopy (TEM) and scanning electron microscopy (SEM). the quantity offers transparent, concise directions on processing organic specimens and contains dialogue at the underlying rules of nearly all of the tactics awarded. A notes part permits effective variation and troubleshooting of protocols.

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Extra info for Electron Microscopy: Methods and Protocols 2nd Edition (Methods in Molecular Biology Vol 369)

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Res. 12, 22–45. 19. Bennett, H. S. and Luft, J. H. (1959) s-Collidine as a basis for buffering fixatives. J. Biophysic. Biochem Cytol. 6, 113–114. 20. Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137a. 21. Schultz, R. L. and Case, N. M. (1970) A modified aldehyde perfusion technique for preventing certain artifacts in electron microscopy. J. Microsc. (Oxford) 92, 69–84. 22. Ericsson, J. L. , Saladino, A. , and Trump, B.

369 Electron Microscopy: Methods and Protocols, Second Edition Edited by: J. , Totowa, NJ 19 20 Mascorro and Bozzola infiltrated with a liquid resin, which is hardened by applying gentle heat (7,8). Hardened resin blocks containing the tissue specimens are thinly sectioned, and the sections treated with solutions of heavy metals in order to impart contrast (9,10). It is only at this point that the thin sections can be placed in the electron microscope column for observation and study. The purpose of chemical fixation is to stabilize the tissue so that it will present a final appearance not substantially unlike its original form.

6, 113–114. 20. Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137a. 21. Schultz, R. L. and Case, N. M. (1970) A modified aldehyde perfusion technique for preventing certain artifacts in electron microscopy. J. Microsc. (Oxford) 92, 69–84. 22. Ericsson, J. L. , Saladino, A. , and Trump, B. F. (1965) Electron microscopic observations of the influence of different fixatives on the appearance of cellular ultrastructure.

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