By Ismael Cotte-Rodriguez, Zhixin Miao (auth.), Guodong Chen (eds.)
This ebook highlights present techniques and destiny tendencies within the use of mass spectrometry to represent protein remedies. As probably the most usually applied analytical suggestions in pharmaceutical learn and improvement, mass spectrometry has been frequent within the characterization of protein therapeutics as a result of its analytical sensitivity, selectivity, and specificity. This booklet starts with an outline of mass spectrometry strategies as concerning the research of protein therapeutics, structural identity options, quantitative methods, by way of experiences regarding characterization of procedure comparable protein drug impurities/degradants, metabolites, greater order constructions of protein therapeutics. either normal practitioners in pharmaceutical examine and experts in analytical sciences will reap the benefits of this publication that info step by step ways and new concepts to unravel demanding difficulties regarding protein therapeutics learn and development.
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Extra resources for Characterization of Protein Therapeutics using Mass Spectrometry
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McEwen CN, McKay RG, Larsen BS (2005) Analysis of solids, liquids, and biological tissues using solids probe introduction at atmospheric pressure on commercial LC/MS instruments. Takáts Z, Czuczy N, Katona M et al (2006) Jet desorption ionization. Haddad R, Sparrapan R, Eberlin MN (2006) Desorption sonic spray ionization for (high) voltage-free ambient mass spectrometry. Grimm RL, Beauchamp JL (2005) Dynamics of field-induced droplet ionization: timeresolved studies of distortion, jetting, and progeny formation from charged and neutral methanol droplets exposed to strong electric fields.
Ferguson C, Benchaar S, Miao Z, Loo J, Chen H (2011) Direct ionization of large proteins and protein complexes by desorption electrospray ionization-mass spectrometry. Hutchens TW, Yip TT (1993) New desorption strategies for the mass spectrometric analysis of macromolecules. Northen TR, Yanes O, Northen MT et al (2007) Clathrate nanostructures for mass spectrometry. Woo HK, Northen TR, Yanes O et al (2008) Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis.
Intact protein ions can also be efficiently and selectively cleaved at tyrosine or histidine residues in gas phase upon the UV irradiation (266 nm) after iodination modification [273]. Modification of tyrosine to iodo-tyrosine followed by UVPD of the carbon–iodine bond generated a radial site specifically at the modified residue. The subsequent dissociation of the protein is largely dominated by radicaldirected reactions, such as the backbone fragmentation at the modified tyrosine site. This method is very useful in terms of reducing the database searching time by several orders of magnitudes [273].