By Branko Braam, Hans Bluyssen (auth.), Gerard Pasterkamp, Dominique P. V. de Kleijn (eds.)
Cardiovascular study: New applied sciences, equipment & Applications serves as a necessary reference fabric supplying a whole assessment of cutting edge new applied sciences, equipment, and purposes in present cardiovascular learn. Cardiovascular examine: New applied sciences, tools & Applications provides a equipment and functions method of cardiovascular learn written by means of well-liked foreign researchers offering commissioned summaries of leading edge research.
Key Topics:
Offers a tools and functions method together with, Cardiovascular tools in:
-Microarrays
-Proteomics
-Real Time PCR
-RNA Interference
-Plus More!
Cardiovascular purposes in:
-Stems Cells and Cardiomyocytes
-Stem Cells and Angiogenesis
-Gene Therapy
-Manufacturing the substitute Blood Vessel
-Closed Chest robot Surgery
-RNA Interference
-Plus More!
Cardiovascular examine: New applied sciences, equipment & Applications is vital for all cardiovascular examine lecturers, bench scientists, and execs wishing to use the newest and maximum within the always rising box of cardiovascular technology.
About the Editors:
Gerard Pasterkamp, PhD, MD is affiliate Professor and Head of Experimental Cardiology Laboratory on the center Lung Centre Utrecht on the collage clinical heart Utrecht (UMCU). Dr. Pasterkamp got his MD at loose college Amsterdam, his PhD on the scientific school on intravascular ultrasound, and his MSc medical Epidemiology on the NIHES Rotterdam. Dr. Pasterkamp's many unique papers and invited reports related to vascular home improvement exhibit his expert curiosity in arterial home improvement in atherosclerotic affliction.
Dominique de Kleijn, PhD is a molecular biologist at Interuniversity Cardiology Institute of the Netherlands (ICIN) and is situated on the Laboratory of Experimental Cardiology on the UMCU. Dr. Klein earned his PhD molecular biology on the Nijmegen college on the division of Molecular Animal body structure, and is well-known as knowledgeable in matrix biology and innate immunity in heart problems.
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Extra resources for Cardiovascular Research: New Technologies, Methods, and Applications
Example text
Clustering has since been applied successfully in several experiments, and 26 Branko Braam and Hans Bluyssen A B R1 R2 6 a 1 5 5 b 1 1 c 2 5 d 2 1 e 3 5 f 3 1 Ratio 2 Gene a c e b d f 1 2 3 4 3 2 1 0 0 4 Ratio 1 0 0 1 2 2 f e d b c a f d b e c D Complete linkage Distance Distance a C Simple linkage 4 6 3 8 4 10 FIGURE 8. Illustration of the basics of cluster analysis. has pointed to interesting groups of coregulated genes. Nevertheless, we feel that the application of cluster analysis is overemphasized.
This is essentially a comparative hybridization experiment. Thus, by comparison of the hybridization of cDNA from a control individual with that from a patient with hypertension, labeled with Cy3 or Cy5 for detection, the relative amounts of all the genes present on the array and expressed in the two samples can be measured. In the photolithographically synthesized array experiments, the two samples under comparison are labeled with the same dye and individually hybridized to different arrays (Fig.
NO can also directly activate or repress the activity of transcription factors, such as NF-κB (Bogdan, 2001), most likely by nitrosylation. Finally, NO can interfere with the action of other signaling cascades (Schindler and Bogdan, 2001). Briefly, the net response of this activation is anti-inflammation, antiadhesion, and antiproliferation. Sequential intervention in the signaling cascade can be performed to decipher which part of signaling cascade is responsible for which set of gene activation, and regulatory motifs can then be searched for in the promoter areas of the genes of interest.