By Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan (auth.), Kannan Balasubramanian, Marko Burghard (eds.)
Due to their infrequent blend of excessive chemical balance, remarkable optical and electric houses, excessive surface-to-volume ratio, and excessive element ratio, carbon nanotubes (CNTs) have made a major impression on fabrics technological know-how, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: tools and Protocols offers trustworthy, constant protocols at the software of CNTs in molecular biology-related fields. those are of significant value, because the commercially to be had CNTs fluctuate in purity, agglomeration nation, in addition to size and diameter distribution, all of that have a profound impression at the dispersability and floor houses of the tubes. the quantity comprises unique sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, supplied by way of specialist researchers from numerous fields. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters contain introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and notes on troubleshooting and heading off recognized pitfalls.
Authoritative and state of the art, Carbon Nanotubes: equipment and Protocols serves to give a contribution to the success of universal criteria and is helping researchers to prevent discrepancies in destiny biology-related CNT studies.
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4. EtONH4 (Sigma-Aldrich). 5. 4. 3. Linkage of DNA Strands to MWCNTs 1. A pair of primers, HBB-1 (5¢ Amine-AGGGTTGGCCAAT CTACTCC-3¢) and HBB-2 (5¢ Amine-TCTCCCCTTCCTA TGACATGA-3¢) (Invitrogen). 2. PCR reaction solution containing genomic DNA, the pair of primers, reaction buffer, MgCl2, dNTPs, and Taq DNA polymerase (Roche, Basel Switzerland). 3. Biophotometer (Eppendorf, Germany). 4. Equipment 1. ULVAC-PHI 5802 XPS system (Kanagawa, Japan). 2. Multimode atomic force microscope (Veeco Instruments).
I water for three times, the mixture was placed in a 55°C oven for 15 min to form the amine-terminated nanotubes. 1. Scheme showing the steps involved in the fabrication of covalently linked DNAnanotube adducts. Reprinted with the permission from ref. (14), copyright 2005 American Institute of Physics 22 Chen et al. 3. Linking DNA strands to the nanotube requires specially prepared DNA strands. An amine-terminal labeled 684 base pairs DNA was amplified from human b-hemoglobin (HBB) gene by polymerase chain reaction (PCR).
N-hydroxysuccinimide (NHS) (Sigma-Aldrich). 4. EtONH4 (Sigma-Aldrich). 5. 4. 3. Linkage of DNA Strands to MWCNTs 1. A pair of primers, HBB-1 (5¢ Amine-AGGGTTGGCCAAT CTACTCC-3¢) and HBB-2 (5¢ Amine-TCTCCCCTTCCTA TGACATGA-3¢) (Invitrogen). 2. PCR reaction solution containing genomic DNA, the pair of primers, reaction buffer, MgCl2, dNTPs, and Taq DNA polymerase (Roche, Basel Switzerland). 3. Biophotometer (Eppendorf, Germany). 4. Equipment 1. ULVAC-PHI 5802 XPS system (Kanagawa, Japan). 2. Multimode atomic force microscope (Veeco Instruments).