By Willis A. Wood (Eds.)
The severely acclaimed laboratory ordinary, Methods in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. considering that 1955, each one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable this day - actually an important e-book for researchers in all fields of lifestyles sciences
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Extra resources for Carbohydrate Metabolism - Part D
Sample text
Shinagawa, O. Adachi and M. Ameyama, J. Biochem. (Tokyo) 85, 1173 (1979). r K. Matsushita, E. Shinagawa, and M. Ameyama, this volume [31]. E. Shingawa, K. Matsushita, O. Adachi, and M. Ameyama, Agric. Biol. Chem. 42, 2355 (1978). 9 E. Shinagawa, K. Matsushita, O. Adachi, and M. Ameyama, Agric. Biol. Chem. 45, 1079 (1981). ~o E. Shinagawa, K. Matsushita, O. Adachi, and M. Ameyama, Agric. Biol. Chem. 46, 135 (1982). 11 M. Ameyama, E. Shinagawa, K. Matsushita, and O. Adachi, Agric. Biol. Chem. 45,851 (1981).
9 p. K. Tubbs a n d G. D. Greville, Biochim. Biophys. Acta 34, 290 (1959). 2o M. G, Waters, M. J. Rispler, E. S. Kline, and R. B. Brandt, Va. J. Sci. 32, 138 (1981). 2~ R. B. Brandt, S. A, Siegel, M. G. Waters, and M. H. Bloch, Anal. Biochem. 102, 39 (1980). [6] D E T E R M I N A T I O N OF D-LACTATE IN PLASMA 37 ml. The assay stock solution for D-lactate determination was prepared daily and contained 50 mg of NAD +, 10 ml of glycine-hydrazine buffer, and 20 ml of distilled water. 75 mM D-lactate), stored at 4° and diluted for use in distilled water.
Discussion The assay described here was directed toward D-lactate concentrations that would be below those expected for the normal glycolytic product, L-lactate. 2 mM. The assay procedure of Gawehn and Bergmeyer 22 was modified, since their procedure was for food or microbiological samples of higher D-lactate concentration. An automated procedure for D- and L-lactate 2~ was also directed to 200-500 times the concentration found in plasma. In order to measure D- and L-lactate in plasma, it is recommended that separate blood samples be collected for 2~ K.